Some variable number of tandem repeat(VNTR) DNA markers show so much variation within population that are coming into side wide use for paternity testing, individual identification, determination of genetic linkage, and more sensitive clinical
and
epidemiological studies of various diseases and cancers. The usual technique for determining genetypes at VNTR loci, the Southern blot assay, requires several days, the use of radioactive isotopes, and rather large amount of DNA being tested. The
recent
development of the polymerase chain reaction(PCR) for amplifying DNA sequences has allowed the possibility of VNTR polymorphism analysis without such difficulties with rapidity, and accuracy.
The analyses of polymorphism, size distribution, and heterozygosity index of VNTR loci were made by PCR on 53 cases of uterine myoma at the department of obstetrics and gynecology, Yeungnam university hospital from September 1994 to December
1994.
The
loci used were apoB, MCT 118, and COL2A1. The size distributions of apoB, MCT 118, COL2A1 were 690~850bp, 500~720bp, and 650~720 bp, respectively. The heterozygosity indices of APOB, MCT 118, COL2A1 were 66.0%, 67.9%, and 64.2%, respectively.
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